JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801133.1
Database:	nt	Go to original record
Description:	Bauhinia sp. CS-2009 voucher Ecklund s.n. tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia sp. CS-2009
Original size:	1050 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttgatt agtttagtaa agtaatcctt
251   ccatcgaaac tccagaaaag aaaggatcaa agataaacgt atatatacgt
301   atacgtaccg aaatactatc tcaactaaat taatgacgac gctatttttg
351   aatatttata taagaaatga aagatgtaaa tcgattcaaa gttgaggaaa
401   gagtcgaata ttcattcatc aaatcattca ctccatcata atccgataga
451   tcttttgaag aaccgattaa tcggacaaga ataaagatag agtcccattc
501   tacatgtcaa tgctgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details