JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801132.1
Database:	nt	Go to original record
Description:	Bauhinia grandidieri voucher Bruneau 1410 (MT) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia grandidieri
Original size:	1049 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	592	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aattgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacggattt cgtttcatta gtaaagtaat ccttccatcg
251   aaactccaga aaagaaagga tcaaagataa acgtatatat acatatacgt
301   accgaactac tatctcaact aaattaatga cgacaccgaa tctctatttt
351   ttaatattta tataagaaat gaaagatgta aatcgattca aagttgagga
401   aagagtcgaa tattcattca tcaaatcatt cactccatca taatccgata
451   gatcttttga agaaccgatt aatcggacaa gaataaagat agagtcccat
501   tctacatgtc aatgctgaca acaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta aaaatcggga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details