JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801118.1
Database:	nt	Go to original record
Description:	Phanera outimouta voucher Redden 1021 (US) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Schnella outimouta
Original size:	1081 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	581	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaaa agagttcaaa
151   taaaaaggat aggtgcagag actcaatgga agctgttcta acaaatggag
201   ttgacgggat ttcgtttcgt tagtaaagta gtaatccttc catcgaaact
251   ccagaaaaga aaggatcaaa gataaacgta tatatacaga tacgtaccga
301   aatactatct caactaaatt aatgacgacg ccgaatctct attttttcat
351   atttataatg aaagatgtaa atggattcaa agttgaggaa aaagtcgaat
401   attcattcat caaatcattc actccatcat aatccgatag atcttttgaa
451   gaactgatta atcggacaag aataaagata gagtcccatt ctacatgtca
501   atactgacaa caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
551   aaatcgtgag ggttcaagtc cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details