JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801115.1
Database:	nt	Go to original record
Description:	Bauhinia blakeana voucher Fougere-Danezan 15 (MT) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia blakeana
Original size:	1050 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	595	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtaaagtaa tccttccatc
251   gaaactccag aaaagaaagg atcaaagata aacgtatata tacgtatacg
301   taccgaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   ttttgaatat ttatataaga aatgaaagat gtaaatcgat tcaaagttga
401   ggaaagagtc gaatattcat tcatcaaatc attcactcca tcataatccg
451   atagatcttt tgaagaaccg attaatcgga caagaataaa gatagagtcc
501   cattctacat gtcaatgctg acaacaatga aatttagagt aagaggaaaa
551   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details