JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801109.1
Database:	nt	Go to original record
Description:	Bauhinia subclavata voucher Kajita 94123101 (TUS) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia subclavata
Original size:	1087 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	602	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtaagtaaa gtaatccttc
251   catcgaaact ccagaaaaga aaggattaaa gagaaaggta tatatacgta
301   tacgtaccga aatactatct caactaaatt aatgacgacg ccgaatctct
351   attttttaat atttatataa gaaatgaaag atgtaaatcg attcaaatca
401   aagttgagga aagagtcgaa tattcattca tcaaatcatt cactccatca
451   taatccgata gatcttttga agaaccgatt aatcggacaa gaataaagat
501   agagtcccat tctacatgtc aatgctgaca acaatgaaat ttatagtaag
551   aggaaaatcc gtcgactttc aaaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details