JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801108.1
Database:	nt	Go to original record
Description:	Gigasiphon macrosiphon voucher Royal Botanic Garden Kew ID1990-1508 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Gigasiphon macrosiphon
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatgatgg agttgacgag atttcgtttc gttagtaaag taatccttcc
251   atcgaaactc cagaaaagaa gggatcaaag agaaacgtat atatacgtat
301   acgtatcgaa atactatctc aacgaaattc atgacgacgc cgaatctcta
351   tttttgaata tttatatgag aaatgaaaga tgtaaatcga ttcaaagttg
401   aggaaaaagt cgaatattca ttcatcaaat cgttcactcc atcataatcc
451   gatagatctt ttgaagaact gattaatcgg acaagaataa agatagagtc
501   ccattctaca tgtcaatact gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details