JRC GMO-Amplicons | EURL GMFF

Record ID:	AB070921.1
Database:	nt	Go to original record
Description:	Taraxacum officinale chloroplast DNA, tRNA-Thr, trnT-trnL intergenic spacer, tRNA-Leu, trnL-trnF intergenic spacer, tRNA-Phe region.
Taxon:	Taraxacum officinale
Original size:	1488 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	599	1125	+	527 bp

Amplicon:

527 bp

1     cgaaatcggt agacgctacg gacttaatag gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aataaaaaag gataggtgca gagactcgat ggaagctgtt
201   ctaacgaatg gagtttattg tcttacgtta atagaggaat ccttctatcg
251   aaacttcaga aaagatgaag gataaacctg tagacataat gtagacataa
301   tacagaagaa ttgttgtcaa tcgattccat attgaagaaa gaatcgaata
351   ttcattgata aaaccattca ctccataatc tgatagacct tttgaagaac
401   tgattagtcg gacgagaata aagatagagt cccgttctac atgtcaatac
451   cggcaacaat gaaatttata gtaagaggaa aatccgtcga tttcaaaaat
501   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	527	508	2.37e-08

Match details