JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801076.1
Database:	nt	Go to original record
Description:	Barklya syringifolia voucher Wiecek 647 (NSW) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Barklya syringifolia
Original size:	1062 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	585	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaaa agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcgtt agtaaagtag taatccttcc
251   aactccagaa aagaaaggat caaagataaa cggatatata catatactta
301   ccgaaatact atctcaacta aattactgac gacgcctaat ctcttttttt
351   taatatttat aatgaaagat gtaaatggat tcaaagttga ggaaaaagtc
401   gaatattcat tcatcaaatc attcactcca tcataatccg atagatcttt
451   tgaagaactt attaatcgga caagaataaa gatagagtcc cattctacat
501   gtcaatactg acaacaatga aatttatagt aagaggaaaa tccgtcgact
551   ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details