JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801064.1
Database:	nt	Go to original record
Description:	Bauhinia natalensis voucher Kirstenboch Botanic Garden 408/83 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia natalensis
Original size:	1074 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgaaaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtgatgtaa tccttccatc
251   gaaactccag aaaagaaagg atcaaagata aacgtatata tacgtatacg
301   tatcgaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   ttgaatattt atataagaaa tgaaagatgt aaatcgattc aaagttgagg
401   aaagagtcga atattcattc atcaaatcat tcactccatc ataatccgat
451   agatcttttg aagaacctat taatcggaca agaataaaga tagagtccca
501   ttctacatgt caatgctgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details