JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801052.1
Database:	nt	Go to original record
Description:	Bauhinia bohniana voucher Douglas 766 (MEL) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia bohniana
Original size:	1060 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaatttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagtgagaat aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtaaagtaa tccttctatc
251   gaaactccag aaaagaaagg atcaaagata aacgtatata tacgtatacg
301   taccgaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   tttttgaatt tttatataag aaatgaaaga tgtaaatcga ttcaaagttg
401   aggaaagagt caaatattca ttcatcaaat cattcactcc atcataatcc
451   gatagatctt ttgaagaacc gattaatcgg acaagaataa agatagagtc
501   ccattctaca tgtcaatgct gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details