JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ801049.1
Database:	nt	Go to original record
Description:	Bauhinia sp. CS-2009 voucher Pennington 768 (MO) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; plastid.
Taxon:	Bauhinia sp. CS-2009
Original size:	1084 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	602	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agagttcaaa
151   aagttagaat aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgacgggatt tcgtttcatt agtaaagtaa tccttccatc
251   gaaactccag aaaagaaagg atcaaagata aacgtatata tacgtatacg
301   taccaaaata ctatctcaac taaattaatg acgacgccga atctctattt
351   atgaatattt atataagaaa tgaaagatgt aaatcgattc aaatcaaagt
401   tgaggaaaga gtcgaatatt cattcatcaa atcattcact ccatcataat
451   ccgatagatc tatcttttga agaaccgatt aatcggacaa gaataaagat
501   agagtcccat tctacatgtc aatgctgaca acaatgaaat ttatagtaag
551   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details