JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ307311.1
Database:	nt	Go to original record
Description:	Moraea verecunda chloroplast partial trnL-trnF intergenic spacer, specimen voucher Goldblatt 7404 MO
Taxon:	Moraea verecunda
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	575	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccccgg aaaaaaaagg
101   ggcaatcctg agccaaatct ttattttgag aaaacaaaca agggttttaa
151   aaactagaat aaaaagggat aggtgcagag actcaatgga agctgttcta
201   acgaatggag ttgactacgt tacgttggta gcgagaatcc ttctatcgaa
251   attaaagaaa ggatgatcct atatatctaa tacgtagtac gtatacatac
301   taacatatca aacgattaat tacgacctga atctattata tattatatat
351   gcaaaattca gagctattgt ggatctagtc caatcgaagt tgaaggaaga
401   atcgatcggt gatcaaatca ttcattccag agtttgatag atcttttgaa
451   aaacgaatta atcggaagag aataaagaga gagtcccgtt ctacatgtca
501   ataccgacaa caatgaaatt tatagtaaaa ggaaaatccg tcgactttag
551   aaatcgttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details