JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ307310.1
Database:	nt	Go to original record
Description:	Moraea verdickii chloroplast partial trnL-trnF intergenic spacer, specimen voucher Bidgood et al. 3795 K
Taxon:	Moraea verdickii
Original size:	1015 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	584	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaacccggg aaaaaaaagg
101   ggcaatcctg agccaaatct ttattttgag aaaacaaaca aggttttaaa
151   aactagaata aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatggggt tgactacgtt acgttggtag cgagaatcct tctatcgaaa
251   ttaaagaaag gatgatccta tatatctaat acatagtacg tatacatact
301   aacatatcaa acgattaatt acgacctgaa tctattatat atttattata
351   tatgcaaaat tcagagctat tgtggatcta ttccaatcga agttgaagga
401   agaatcgatc ggtgatcaaa tcattcattc cagagtttga tagatctttt
451   gaaaaacgaa ttaatcggaa gagaataaag agagagtccc gttctacatg
501   tcaataccga cagcaatgaa atttatagta aaaagaaaat ccgtcgactt
551   tagaaatcgt gagggttcaa gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details