JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ466484.1
Database:	nt	Go to original record
Description:	Trattinnickia cf. burserifolia Hoffman et al. 694 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Trattinnickia cf. burserifolia Hoffman et al. 694
Original size:	1034 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	596	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcca tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcgatcct gagccaaatc ctgttttacg agaacaaaca agggttcaaa
151   ggggataggt gcagagactc aatggaagct gttctaacaa atggagttga
201   ctgccttttt ttgggaaaga aaggaaaatg aatccttata tccaatatcg
251   aaactccata aaggatgaag gataagccta tatacactac gtatacgtaa
301   tgaaaaacta tctcaaaaat gacgaccaga atccgtattt tttttatgaa
351   aaaaaaaaaa gaaaagaatt gttgggaatc gattccaagt tgaagaacga
401   attgaatatt cattgatcaa atcattcact ccatagtctg atcgatcttt
451   tcttttgaag aacttattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa tagcaatacc ggcaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details