JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ466481.1
Database:	nt	Go to original record
Description:	Protium madagascariense tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Protium madagascariense
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatcaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaacaaaca agggttcaga
151   acacgagaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgccttttt gtgggaaaga aaggaaaatg aatccttcta
251   tccaatatcg aaactccata aaggatgaag gataagccta tataaactac
301   gtatacgtaa tgaaaaacta tctcaaaaat gacgaccaga atccgtatct
351   tttttttatg aaaaaaaaag aaaagaattg ttgtgaatcg attccaagtt
401   gaagaaagaa tcgaatattc attgatcaaa tcattcactc catagtctga
451   tcgatgtttt cttttgaaga attttttaat caatcggacg agaataaaga
501   tagagtccca ttctacatgt caatagcaat accgacaaca atgaaattta
551   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details