JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ466470.1
Database:	nt	Go to original record
Description:	Canarium littorale tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Canarium littorale
Original size:	1035 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	603	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaacaaaca agggttcaga
151   acacgagaaa ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgccttttt ttgggaaaga aaggaaaatg aatccttcta
251   tccaatttcg aaactccata aaggatgaag gataagccta tatacactac
301   gtatacgtaa tgaaaaacta tctcaaaaat gacgaccaga atccgtattt
351   ttttttatga aaaaaaagaa aagaattgtt gggaatcgat tccaagttga
401   agaaagaatc gaatattcat tgatcaaatc attcactcca tagtctgatc
451   gatcttttct tttgaagaac ttattaatcg gacgagaata aagatagagt
501   cccattctac atgtcaatag caataccggc aacaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details