JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ466468.1
Database:	nt	Go to original record
Description:	Canarium harveyi tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Canarium harveyi
Original size:	1030 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	602	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaacaaaca agggttcaga
151   acacgagaaa ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgccttttt ttgggaaaga aagaaaaatg aatccttcta
251   tccaatatcg aaactccata aaggatgaag gataagccta tatacactac
301   gtatacgtaa tgaaaaacta tctcaaaaat gatgaccaga atccgtattt
351   tttttatgaa aaaaaagaaa agaattgttg ggaatcgatt ccaagttgaa
401   gaaagaatcg aatattcatt gatcaaatca ttcactccat agtctgatcg
451   atcttttctt ttgaagaact tattaattgg acgagaataa agatagagtc
501   ccattctaca tgtcaatagc aataccggca acaatgaaat ttatagtaag
551   aggaaaatcc gtcgacttta gaaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details