JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ307260.1
Database:	nt	Go to original record
Description:	Moraea flaccida chloroplast partial trnL-trnF intergenic spacer, specimen voucher Goldblatt s.n. MO
Taxon:	Moraea flaccida
Original size:	980 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	566	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaacccggg aaaaaaaagg
101   ggcaatcctg agccaaatct ttattttgag aaaacaaaca agggttttaa
151   aaactagaat aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acgaatggag ttgactacgt tacgttggta gtgagaatcc ttctatcgaa
251   attaaagaaa ggatgatcct atatatctaa tacgtagtac gtatacatac
301   taacatatca aacgattaat tacgacctga atctattata tatttattat
351   atatgcaaaa ttcagagcta ttgtggatct attccaatcg aagttgaagg
401   aagaatcgat cggtgatcaa atcattcatt ccagagtttg atagatcttt
451   tgaaaaacga attaatcgga agagaataaa gagagagtcc cgttctacat
501   gtcaataccg acagcaatga aatttatagt aaaaggaaaa tccgtggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details