JRC GMO-Amplicons | EURL GMFF

Record ID:	FJ466463.1
Database:	nt	Go to original record
Description:	Bursera tecomaca tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Bursera tecomaca
Original size:	1033 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	595	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttacg agaacaaaca agggttcaga
151   acacgagaaa ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ttgccttttt ttgggaaaga aaggaaaatg aatccttcta
251   tccaatatcg aaaatccata aaggatgaag gataagccta tatacactac
301   gtatacgtaa tgaaaaacta tctcaaaaat gacgaccaga atccgtatct
351   tttttgatga aaaaaaagaa aagaattgtt gtgaatcgat gaagaaagaa
401   tcgaatattc attgatcaaa tcattcactc catagtctga tcgatctttt
451   cttttgaaga acttattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat agcaataccg gcaacaatga aatttatagt aagaggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details