JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ307253.1
Database:	nt	Go to original record
Description:	Moraea cedarmonticola chloroplast partial trnL-trnF intergenic spacer, specimen voucher Goldblatt 3871 NBG
Taxon:	Moraea cedarmonticola
Original size:	1020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gactttgatt gaattgagcc ttagtatgga
51    aacctgctaa gtggtaactt ccaaattcag agaaacccgg gaaaaaaaag
101   gggcaatcct gagccaaatc tttattttga gaaaacaaac aagggtttta
151   aaaactagaa taaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatgga gttgactacg ttacgttggt agtgagaatc cttctatcga
251   aattaaagaa aggatgatcc tatatatcta atacgtagta cgtatacata
301   ctaacatatc aaacgattaa ttacgacctg aatctattat atatttatta
351   tatatgcaaa attcagagct attgtggatc tattccaatc gaagttgaag
401   gaagaatcga tcggtgatca aatcattcat tccagagttt gatagatctt
451   ttgaaaaacg aattaatcgg aagagaataa agagagagtc ccgttctaca
501   tgtcaatacc gacagcaatg aaatttatag taaaaggaaa atccgtcgac
551   tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details