JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ409410.1
Database:	nt	Go to original record
Description:	Eriochilus cucullatus chloroplast tRNA-Leu gene for transfer RNA-Leu and trnL-F intergenic spacer
Taxon:	Eriochilus cucullatus
Original size:	847 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	547	+	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    cctgctaagt ggtaacttcc aaattcagag aaaccctgga actaaaaatg
101   ggcaatcctg agccaaatct ttgttttgag aaaacaaaaa attcaaaaat
151   gagaataaaa agggataggt gcagagactc gatggaagct gttctaacga
201   atgtacttta ctacattaga ttagagaatc taatatacct aatgtgtacg
251   tatacatact gacatagtaa atcacattta tttctattac ctattactga
301   ttattagtat gagtatagga tactatgaag aatggaagag ttcttgtgaa
351   tcaattctca ttgaaattga aaatattcag tgatcaaatg attcattcca
401   gagtttgata gattttttga agatgaattg gacgagaata aagagagagt
451   cccattttac atgccaatac cgacaacaat gaaatttaga gtaagaggaa
501   aatccgtcga attttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details