JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ409407.1
Database:	nt	Go to original record
Description:	Elythranthera emarginata chloroplast tRNA-Leu gene for transfer RNA-Leu and trnL-F intergenic spacer
Taxon:	Elythranthera emarginata
Original size:	860 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	4	567	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttttaaaaa gaagaaaaag ggataggtgc
151   agagactcaa tggaagctgt tctaacgaat gaaattgact gcgtttgatt
201   agtagctaaa attcttttat caacagaaag gataacctta tatacctata
251   ttactattag gatatgatta ttagtatgag tataggataa ggatatacag
301   aaaccctcga tttctagttg atattaatta gaattataga ctatgaaaaa
351   tggaagagaa ttggagctga aaaaaaagaa ttgaattcaa atattcagta
401   atcgatgatt cattccagag tttgatatat tttttgaaga tgaatcggac
451   gagaataaag agagagtccc attttacatg tcaataccga caacaatgaa
501   atttatagta agaggaaaat ccgtcgaatt tttaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details