JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ409373.1
Database:	nt	Go to original record
Description:	Acianthus exsertus chloroplast tRNA-Leu gene for transfer RNA-Leu and trnL-F intergenic spacer
Taxon:	Acianthus exsertus
Original size:	899 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	583	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttgattg gattggattg agcttggtat
51    ggaacctgct aagtggtaac ttccaaattc agagaaaccc tggaactaaa
101   aatgggcaat cctgagccaa atctttgaaa aacattcaaa atgagaatag
151   aaggggatag gtgcagagac tcaatggaag ctgttctaac gaatgaactt
201   gactacgtta gattagtagc taaaatcctt ctatcaaaag aaaagatcaa
251   aatagcagaa aggataacct tatataccta actgacatag taaacgatta
301   atcacatctc tttctattac atattcctat taggaataga ctatgaaaaa
351   tggaagagtt ctcgtgaatc aattagattt gaagttgaaa aaagaatcaa
401   attcaaatat tcagtgatca aatgattcat tccagagttt gatatatttt
451   ttgaagatta atcggacgag aataaagaga gagtcccatt ttacatgtca
501   ataccggcaa cagggaaatt tagagtaaga ggaaaatccg tcgaattttt
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details