JRC GMO-Amplicons | EURL GMFF

Record ID:	AB054236.1
Database:	nt	Go to original record
Description:	Cinnamomum cassia chloroplast trnL gene, intron, specimen_voucher:#015.
Taxon:	Cinnamomum aromaticum
Original size:	553 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	553	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttggttg gattgagcct tggtatggaa
51    acctactaag tgataacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaggg ttcagaaagc
151   gagaaccaaa aaaaggatag gtgcagagac tcaaaggaag ctgttctaac
201   gaatggagtt gattaacatt ggtataggaa tccttctatc gaaattccag
251   aaaggatgac cctatcctat atacgtactg aaatatcaaa caattaatca
301   cgatccgatt ccgtattttt ttgatatgaa aaatggaaga attcttgtga
351   atcgattcca aattgaagga agaatcgaat attcagtgat caaatcattc
401   actcctcgga tagatctttt gaagaactga ttaatcggac gagaataaag
451   atagagtcca ttctacatgt caataccgac aacaatgaaa tttatagtaa
501   ggggaaaatc cgtcgacttt agaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details