JRC GMO-Amplicons | EURL GMFF

Record ID:	EU792463.1
Database:	nt	Go to original record
Description:	Deschampsia antarctica voucher Peterson et al. 17079 US trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Deschampsia antarctica
Original size:	1739 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	775	1387	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc catgttttga gaaaacaagg gggttctcga
151   actagaatgc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta agttgtgttg ttagtggaat tccttctcca
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgacatagc
301   aaacgattaa tcacagaacc catattataa tataggttct ttattctttt
351   ttagaatgaa atttgaaata gaaatgatta tgaaataaaa aattcagaat
401   ttttttagaa ttattgtgaa tccattccaa tcgaatattt agtaatcaaa
451   tccttcaatt caaagttttg aggtctttaa aaaagtgtat taatcggacg
501   aggacaaaga gagagtccca ttctacatgt caatactgac aataatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details