JRC GMO-Amplicons | EURL GMFF

Record ID:	EU792450.1
Database:	nt	Go to original record
Description:	Poa kelloggii voucher Soreng 5962 US tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa kelloggii
Original size:	1821 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	793	1402	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct ggtatggaaa
51    cctgctaagt ggtaacttcc aaattcagag aaaccctgga attaaaaaag
101   ggcaatcctg agccaaatcc gtgttttgag aaaacaaggg ggttctcgaa
151   ctagaataca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatct aattaattac gttgtgttgt tagtggaatt ccttctaaat
251   tcgaaaaaga agggctttat acatctaata aacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccatatt ataatatagg ttctttattc
351   ttttttagaa tgaaatagaa atgattatga aataaaaaat tcagaatttt
401   tttagaatta ttgtgaatcc attccaatcg aatatttagt aatcaaatcc
451   ttcaattcaa agttttgagg tctttaaaaa agtggattaa tcggacgagg
501   acaaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details