JRC GMO-Amplicons | EURL GMFF

Record ID:	EU792439.1
Database:	nt	Go to original record
Description:	Apera interrupta voucher Peterson et al. 19173 US tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Apera interrupta
Original size:	1830 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	865	1485	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacctatat tataatatat ggttctttat
351   tcttttttag aatgaaattt gaaatagaaa tgattatgaa ataaaaaatt
401   cataattttt ttttagaatt attgtgaata cattccaatc gaatatttag
451   taatcaaatg cttccattca aagtttttag gtctttaaaa aagtggatta
501   atcggacgag aacaaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details