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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: FN377812.1
Database: nt Go to original record
Description: Cloning vector pKIGW containing genes for GUS, LhGR, nptII and ccdB
Taxon: Cloning vector pKIGW
Original size: 19554 bp

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 6352 6547 - 196 bp
Amplicon: 196 bp 
1     gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagaa
51    tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca
101   tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
151   tgtgatatct ccactgacgt aagggatgac gcacaatccc actatc

View other 185 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.45e-06
primerB 100.0 20 bp 0 0 196 177 9.33e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.