JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ296500.1
Database:	nt	Go to original record
Description:	Walafrida nitida chloroplast tRNA-Leu (UAA) gene
Taxon:	Walafrida nitida
Original size:	1751 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	820	1390	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtacggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   ggcaaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttgact tcgttggtag aggaatctct ccatcgaaac ttcagaaagg
251   gtgacgggta aacgtatcta ttgaatactc tatcaaaatc aaatgattaa
301   tggtgccccg aatccgtatc tgtatttttg atctaaaaag tggaaaattt
351   tgtgtgaatt tattccacca ttgaagaaag aattaactaa aatattcatt
401   catcaaatca ttcactccat agtctgatag atctttaaaa gatctgatta
451   attggacaag aataaagata gagtcccatt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details