JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ296506.1
Database:	nt	Go to original record
Description:	Tetraselago longituba chloroplast tRNA-Leu (UAA) gene
Taxon:	Tetraselago longituba
Original size:	1836 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	888	1466	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtacggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ttcaaaacaa aggttcagaa
151   ggcaaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttgact tcgttggtag aggaatctct ccatcgaaac ttcagaaagg
251   gtgacgggta aacgtatcta ttgaataccc tatcaaaatc aaatgattaa
301   tggtgccccg aatccgtatc tgtatttttg atataaaaag tgaaaaattt
351   ggtgtgaatt tattccacca ttgaagaaag aattaactaa aatattcatt
401   catcaaatca ttcactccat agtctgatag tctgatagat cttttaaaga
451   tctgattaat tggacaagaa taaagataga gtcccatttt acatgtcaat
501   accggcaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details