JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ296505.1
Database:	nt	Go to original record
Description:	Reyemia chasmanthiflora chloroplast tRNA-Leu (UAA) gene
Taxon:	Zaluzianskya chasmanthiflora
Original size:	1958 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	989	1566	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcccgtttt ctcaaaacaa gggttcagaa
151   ggcaaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact tcgttggtag aggaatctct acatcgaaac ttcagaaagg
251   gtgatgggta aacttatcta ttgaatactc tatcaaaatc aaatgattaa
301   tggtgccccg aatctgtatc tgtatttttt atattttata taaaaagtgg
351   aaaatttggt gtgaatttat tccaccattg aagaaagaat taactaaaat
401   attcattcat caaatcattt actccatagt ctgatagatc ttttaaagat
451   ctgattaatt ggacaagaat aaagatagag tcccattcta catgtcaata
501   ccggcaacaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details