JRC GMO-Amplicons | EURL GMFF

Record ID:	EU544024.1
Database:	nt	Go to original record
Description:	Microcnemum coralloides subsp. anatolicum isolate chen 734 trnL-trnT intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Microcnemum coralloides subsp. anatolicum
Original size:	1641 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	594	1205	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaaggaaag
151   gataggtgca gagactcaaa ggaagttgtt ctaacaaatg gatttgggtt
201   gactgtgttg ttctaagaat tcttccgtca aaactccaat ctaaaaaggg
251   taaagaatat actatatcaa atgattaatg acaccccgaa tctattttgt
301   attttttttt tcgaattttt tagtatttat tatttctata tttaatagaa
351   aagatgaaat tctcgctaac taaaaattgc gaatatgaaa tgaaaatata
401   tattatatga ataggaaaaa aacaaaggga agaatttttg tgaatcgatt
451   tcaaattgaa aaagaatcaa atattcattc atcaaatcat tcacttccga
501   gaataaagat agagtcccat tctacatgtc aatactgaca agaatgaaat
551   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details