JRC GMO-Amplicons | EURL GMFF

Record ID:	EU544022.1
Database:	nt	Go to original record
Description:	Microcnemum coralloides subsp. anatolicum isolate chen 609 trnL-trnT intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Microcnemum coralloides subsp. anatolicum
Original size:	1693 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	643	1257	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaaggaaag
151   gataggtgca gagactcaaa ggaagttgtt ctaacaaatg gatttgggtt
201   gactgtgttg ttctaagaat tcttccgtca aaactccaat ctaaaaaggg
251   taaagaatat actatatcaa atgattaatg acaccccgaa tctattttgt
301   attttttttt ttttcgaatt ttttagtatt tattatttct atatttaata
351   gaaaagatga aattctcgct aactaaaaat tgcgaatatg aaatgaaaat
401   atatattata tgaataggaa aaaaacaaag ggaagaattt ttgtgaatcg
451   atttcaaatt gaaaaagaat caaatattca ttcatcaaat cattcactcc
501   cgagaataaa gatagagtcc cattctacat gtcaatactg acaagaatgc
551   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details