JRC GMO-Amplicons | EURL GMFF

Record ID:	EU544019.1
Database:	nt	Go to original record
Description:	Microcnemum coralloides subsp. anatolicum isolate chen 1354 trnL-trnT intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Microcnemum coralloides subsp. anatolicum
Original size:	1642 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	594	1206	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaaggaaag
151   gataggtgca gagactcaaa ggaagttgtt ctaacaaatg gatttgggtt
201   gactgtgttg ttctaagaat tcttccgtca aaactccaat ctaaaaaggg
251   taaagaatat actatatcaa atgattaatg acaccccgaa tctattttgt
301   attttttttt ttcgaatttt ttagtattta ttatttctat atttaataga
351   aaagatgaaa ttctcgctaa ctaaaaattg cgaatatgaa atgaaaatat
401   atattatatg aataggaaaa aaacaaaggg aagaattttt gtgaatcgat
451   ttcaaattga aaaagaatca aatattcatt catcaaatca ttcactcccg
501   agaataaaga tagagtccca ttctacatgt caatactgac aagaatgaaa
551   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details