JRC GMO-Amplicons | EURL GMFF

Record ID:	EU544015.1
Database:	nt	Go to original record
Description:	Microcnemum coralloides subsp. coralloides isolate chen 642 trnL-trnT intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Microcnemum coralloides subsp. coralloides
Original size:	1592 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	538	1153	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattcaaaaa
101   aaaaagggca atcctgagcc aactcctttt ttcaaaataa aaaaaaagga
151   aaggataggt gcagagactc aaaggaagtt gttctaacaa atggatttgg
201   gttgactgtg ttgttgtaag aattcttccg tcaaaactcc aatctaaaaa
251   gggtaaagaa tatactatgt caaatgatta atgacacccc gaatctattt
301   tgtatttttt ttttttttcg aattttttag tatttattat ttctatattt
351   aatagaaaag atgaaattct cgctaactaa aaatttcgaa tatgaaatga
401   aaatatatat tatatgaata ggaaaacaaa gggaagaatt tttgtgaatc
451   gatttcaaat tgaaaaagaa tcaaatattc attcatcaaa tcattcactc
501   ccgagaataa agatagagtc ccattctaca tgtcaatact gacaagaatg
551   caatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details