JRC GMO-Amplicons | EURL GMFF

Record ID:	EU544014.1
Database:	nt	Go to original record
Description:	Arthrocnemum macrostachyum isolate chen 353 trnL-trnT intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Arthrocaulon macrostachyum
Original size:	1687 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	656	1254	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaggatagg
151   tgcagagact caaaggaagt tgttctaaca aatgggtttg ggttgactgt
201   gttgttctaa gaattcttcc gtcaaaactc caatctaaaa agggtgaaga
251   atatactata tcaaatgatt aatgacaccc cgaatctatt ttgtattttt
301   ttctaatttt ttagtattta ttatttctat atttaataga gaagatgaaa
351   ttctcgctaa ctaaaaattg cgaatatgaa atgaaaatat atattatatg
401   aataggaaaa aaagggaaga atttttgtga atcgatttca aattgaaaaa
451   gaatcaaata ttcattcatc aaatcattca ctcccgagaa taaagataga
501   gtcccattct acatgtcaat actgacaaga atgaaattta tagtaagagg
551   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details