JRC GMO-Amplicons | EURL GMFF

Record ID:	EU544013.1
Database:	nt	Go to original record
Description:	Arthrocnemum macrostachyum isolate chen 2002/7 trnL-trnT intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Arthrocaulon macrostachyum
Original size:	1689 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	657	1256	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaggatagg
151   tgcagagact caaaggaagt tgttctaaca aatgggtttg ggttgactgt
201   gttgttctaa gaattcttcc gtccaaactc caatctaaaa agggtgaaga
251   atatactata tcaaatgatt aatgacaccc cgaatctatt ttgtattttt
301   tttctaattt tttagtattt attatttcta tatttaatag agaagatgaa
351   attctcgcta aataaaaatt gcgaatatga aatgaaaata tatattatat
401   gaataggaaa aaaagggaag aatttttgtg aatcgatttc aaattgaaaa
451   agaatcaaat attcattcat caaatcattc actcccgaga ataaagatag
501   agtcccattc tacatgtcaa tactgacaag aatgaaattt atagtaagag
551   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details