JRC GMO-Amplicons | EURL GMFF

Record ID:	EU401427.1
Database:	nt	Go to original record
Description:	Centrolobium tomentosum voucher Walter, B.M.T. 3343 (NY) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Centrolobium tomentosum
Original size:	1083 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	612	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgagaacttt caaattcaga gaaaccccgg aattaacaat
101   gggcaatcct gagccaaatc ccgttttccg aaagcaaaga aaagttaaga
151   aagcgagaat aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgacgaca tttcctttcg cgttaggaaa ggaatccttc
251   catcgaaatt ctagaaagga aaggatatat atatatgtat atatacgtat
301   ttgtattgaa atactatttc aattgagtaa tgaagactcc actatttgtt
351   actctttata tcacatttat atcacaaatg acaaatgaaa aatgtgaatc
401   aaatcaaatc caagttgaag aaagaattga atattcactg atcaaatcat
451   tcactccatc atagtctgat agatcttttg aagaactgat taatcggacg
501   agaataaaga tagagtccca ttctacatgt caataccgac aacaatgaaa
551   tttatagtaa gaggaaaatc cgtcgactta ataaatcgtg aggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details