JRC GMO-Amplicons | EURL GMFF

Record ID:	EU817471.1
Database:	nt	Go to original record
Description:	Ixora tanzaniensis trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ixora tanzaniensis
Original size:	1672 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	759	1324	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggtacagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagcatg ttctaacaaa
201   tggagttggc tgcgttagta gagaaacctt tccatcgaaa attccgaaag
251   gataaagtga aagataaagg tatatacgta ttgaatacta tatcaaatga
301   ttaatgacga ctcgaatgaa tctgtatttt ttatatgaaa agggaagaat
351   tggtgtgaat agattccaca ttaaagaaag aatcgaatat tcattgatta
401   aatgattcac tccatagtct gatagatctt ttcaagaatt gattaatcgg
451   acgagaataa agatagagcc ccgttctaca tgtcaatgtc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details