JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ296511.1
Database:	nt	Go to original record
Description:	Jamesbrittenia megadenia chloroplast tRNA-Leu (UAA) gene
Taxon:	Jamesbrittenia megadenia
Original size:	1636 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	688	1260	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacct caaaacaaag
151   gttcagaagg cgaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgca ttggtagagg aatctttcca tcgaaacttg
251   agaaagggtg acggataaat gtctctattg aatactatag caaaatcaaa
301   tgattaatga tggcccgaat ctctattttt ttatatgaaa aatggaaaag
351   ttgatatgaa tttattctat nattgaagaa agaatcaaat aaaatattca
401   ttcatcaaat cattcactcc atagtctgat agatctttta aagaactgat
451   taatcggacg agaataaaga tagagtccca ttctacatgt caataccgac
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt naaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details