JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ296514.1
Database:	nt	Go to original record
Description:	Camptoloma lyperiiflorum chloroplast tRNA-Leu (UAA) gene
Taxon:	Camptoloma lyperiiflorum
Original size:	1668 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	711	1276	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   tgcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   gtgacggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   cccgaatctt tatctgtatt tttttatatg aaaaatggaa aaattggtgt
351   gaatttattc cacnattgaa gaaagaatca aataaaatat tcattcatca
401   aatcattcac tccatagtct gatagatctt ttaaagaact gattaattgg
451   acgagaataa agatagagtc ccattctaca tgtcaatacc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details