JRC GMO-Amplicons | EURL GMFF

Record ID:	AM117366.1
Database:	nt	Go to original record
Description:	Keetia gueinzii chloroplast tRNA-Leu gene, trnT-trnL intergenic spacer and trnL-trnF intergenic spacer, specimen voucher Bremer et al. 4349 (UPS)
Taxon:	Keetia gueinzii
Original size:	1517 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	614	1163	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaacctgga attaataaaa
101   aggggcaatc ctgaccaaat cctgttttcc gaaaccaaag gttcagaaag
151   tgaaaaaggg ataggtgcag agactcaatg gaagctgttc taacaaatgg
201   agttggctgc gttagtagag aaatctttcc atcgaaaatt ccgaaaggat
251   aaaacgtata cgtattgaat actatatcaa atgattaatg acgactcgac
301   tgaatctgta ttttttatat gaaaatggaa gaattggtgt gaatagattc
351   cacattgaag aaagaatcga atattcattg attaaatgat tcactccata
401   gtctgataga tcttttcaag aattgattaa tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa tgtcggcaac aatgaaattt atagtaagag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details