Match details

Record ID: AM117353.1
Database: nt Go to original record
Description: Coddia rudis chloroplast tRNA-Leu gene, trnT-trnL intergenic spacer and trnL-trnF intergenic spacer, specimen voucher Bremer et al. 4279 (UPS)
Taxon: Coddia rudis
Original size: 1612 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 713 1285 + 573 bp

Amplicon: 573 bp
1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat tctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catcgaaaat tccgaaagga
251   taaagtataa agtgaaggat aaacgtatat atacgtattg aatactatat
301   caaatgatta atgacgactc gactgaatct gtatttttta tatgaaaacg
351   gaagaattgg tgtgaataga ttccacattg aagaaagaat cgaatattca
401   ttgatcaaat gattcactcc atagtctgat agatcttttc aagaattgat
451   taatcggacg agaataaaga tagagtcccg ttctacatgt caatgtcggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.58e-08
primerB 100.0 20 bp 0 0 573 554 2.58e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.