JRC GMO-Amplicons | EURL GMFF

Record ID:	AM117350.1
Database:	nt	Go to original record
Description:	Boholia sp. Bicknell 1561A chloroplast tRNA-Leu gene, trnT-trnL intergenic spacer and trnL-trnF intergenic spacer, specimen voucher In cult. Phillipines, Bicknell 1561A
Taxon:	Boholia sp. Bicknell 1561A
Original size:	1416 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	452	1020	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    atacactaag tgataacttt caaattcaga gaaacctgga attaataaaa
101   aggggcaatc ctgagccaaa tcccgttttc cgaaaccaaa ggttcaaaaa
151   gggaaaaagg ataggtgcag agactcaacg gaagctgttc taacaaatgg
201   agttggttgt gttagtagag aaatctttcc atcgaaaatt ccgaaaggaa
251   aaggtgaagg ataaacgtat atatgtattg aatgctatat caaaagatta
301   atgacgactc ggctgaatct gtatttttta tttttatatg aaaacagaag
351   aattggtatg aatagattcc acattgaaga aagaatcgaa tagtcattaa
401   tcaaatgatt cactccatag tctgatagat cttttcaaga attgattaat
451   cggacgagaa taaagataga gtcccgttct acatgtcaat gtcggcaaga
501   atgaaattta tagtaagagg aaaatccgtc gacttttaaa atcgtgaggg
551   ttcaagtccc tctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details