JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660642.1
Database:	nt	Go to original record
Description:	Torreya taxifolia voucher 20070301 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Torreya taxifolia
Original size:	969 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	537	+	537 bp

Amplicon:

537 bp

1     cgaaatcggt agacgctacg gacttaatcg aaatgagcct tggtatggaa
51    acttactaag tgatagcttc caaatccagg gaaccctggg atattctgaa
101   tgggcaatcc tgagccaaat ccctttataa gggatgataa ttaatcattt
151   cccagaaagg gataggtgca gagactcgac ggaagctatt ctaacgaatg
201   aatatcattt gaatttgacc caatactatt atctagagag tgccctctgt
251   atataacact taataaatga aacaaaacta acgattagaa cttgagtcgt
301   tctaggcttt ttttttagga gcctagctca aaatcaattt gaatgaacta
351   attcattaag taatccaatt tagagcttct ttagaaagag agttaattcc
401   atttttttaa tgaatgattg gacgaggata aagatagagt ccaattctac
451   atgtcaatac caacaacaat gcaaattgca gtaggaggaa aatccgttgg
501   ttttatagac cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	537	518	2.42e-08

Match details