JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660640.1
Database:	nt	Go to original record
Description:	Torreya fargesii voucher WH20070101 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Torreya fargesii
Original size:	967 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	536	+	536 bp

Amplicon:

536 bp

1     cgaaatcggt agacgctacg gacttaatcg aattgagcct tggtatggaa
51    acttactaag tgatagcttc caaatccagg gaaccctggg atattctgaa
101   tgggcaatcc tgagccaaat ccctttataa gggatgataa ttatcatttc
151   ccagaaaggg ataggtgcag agactcgacg gaagctattc taacgaatga
201   atatcatttg aatttgaccc aatactatta tctagagagt gccctctgta
251   tataacactt aataaatgaa acaaaactaa cgattagaac ttgagtcgtt
301   ctaggctttt tttttaggag cctagctcaa aatcaaattg aatgaactaa
351   ttcattaatt aatccaattt agagcttctt tagaaagaga gttaattcca
401   tttttttaat gaatgattgg tcgaggataa agatagagtc caattctaca
451   tgtcaatacc aacaacaatg caaattgcag taggaggaaa atccgttggt
501   tttatagacc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	536	517	2.41e-08

Match details