JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660629.1
Database:	nt	Go to original record
Description:	Cephalotaxus hainanensis voucher DZ200612 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Cephalotaxus hainanensis
Original size:	828 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	544	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tagtatggaa
51    acctactaag tgatagcttc caatgcaggg aaccctggga tattttgaat
101   gggcaatcct gagccaaatc cggtttatag aaacatagtt ttctttccta
151   gaaaaggata ggtgcagaga ctcgacggaa gctattctaa cgaatgaata
201   tcattttaat ttgatcctgt acactatcta cagagtgccc tctgtattta
251   aaaattgaaa aagtgatacc atccaaaaaa caaaactaac gattagaact
301   tgagtcgttc taggcttttt tttttaggag cctagcttcg aagtgaaggt
351   aatgactcat taagtaatcc aattaagagc ttcttttaga aataaagtga
401   attcaattat tggaattaat gattggacga ggataaagat agagtccaat
451   tctacatgtc attgcaataa caacaatgca aattgcagta kgatgaaaat
501   ccgttggttt tatagaccgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details