JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660622.1
Database:	nt	Go to original record
Description:	Amentotaxus yunnanensis voucher AF20070402 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Amentotaxus yunnanensis
Original size:	1016 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	551	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaatcg aattgagcct tggtatggaa
51    acttaccaag tgatagcttc caaatccagg gaaccctggg atattctgaa
101   tgggcaatcc tgagccaaat ccggtttata gggacaatag tttcctttcc
151   tagaaagaga taggtgcaga gactcgacgg aagctattct aacgaatgaa
201   tatcatttga atttgaccca atactctatc tagagagtgc cctctagatt
251   taacactttt aaaagtgtta tatactggtt ctaacgatag aacttgagtc
301   gttctaagct ttttttagaa gcctagctca aaatcgaatt gaaggaatac
351   ttaattactt aatagaatta agtattataa tgaagagctt ttctaaaaag
401   agagttaatt acatttttgt aataaatgta attggacgag gataaagata
451   gagtccaatt ctacatgtca ataccaacaa caatgcaaat tgcagtagga
501   ggaaaatccg ttggttttat agaccgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details