JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660620.1
Database:	nt	Go to original record
Description:	Taxus wallichiana voucher CY007 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Taxus wallichiana
Original size:	900 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	524	+	524 bp

Amplicon:

524 bp

1     cgaaatcggt agacgctacg gactttaata gaattgagcc cttggtatgg
51    aaacttacta agtgatagct tccaaataca ggggaaccct ggaatatttt
101   gaatgggcaa tcctgatcca aatccgtatt ataggaacaa taattttatt
151   ttctagaaaa gggataggtg cagagactca acggaagata ttctaacgac
201   ttaatatcat ttgaatttga accaatattc tatctacaga gtgtagtatg
251   ttattgaaaa ctttgaaagt gttctgtatc atcgttaaaa cttgtttcaa
301   cgattagaac ttgagttgtt ctaggcttgc ctagcttaat gaatacttaa
351   ttaaagtaat tcaattaaga aataaataga atttattcta tttttgaatt
401   attggacgag gataaagata gagtccaatt ctacatgtaa atgccaacaa
451   caacaatgca aattgcagta gtcggaaaat ccgttggttt tataaaccgt
501   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	524	505	2.36e-08

Match details