JRC GMO-Amplicons | EURL GMFF

Record ID:	EF660619.1
Database:	nt	Go to original record
Description:	Cephalotaxus oliveri voucher GL200703 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and trnF gene, partial sequence; chloroplast.
Taxon:	Cephalotaxus oliveri
Original size:	830 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	546	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tagtatggaa
51    acctactaag tgatagcttc caaatgcagg gaaccctggg atattttgaa
101   tgggcaatcc tgagccaaat ccggtttata gaaacgtagt tttctttcct
151   agaaaaggat aggtgcagag actcgacgga agctattcta acgaatgaat
201   atcattttaa tttgatcctg tacactatct acagagtgcc ctctgtattt
251   aaaaatttaa aaagtgatac catccataaa acaaaactaa cgattagaac
301   ttgagtcgtt ctaggctttt tttttttagg agcctagctt cgaagtgaag
351   gtaatgactc attaagtaat ccaattaaga gcttatttta gaaataaagt
401   gaattcaatt attggaatta atgattggac gaggataaag atagagtcca
451   attctacatg tcattgcaat aacaacaatg caaattgcag taggatgaaa
501   atccgttggt tttatagacc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details